Test procedure of Sars Cov-2 rapid antigen test Apr 09, 2021

Use Sars Cov-2 rapid antigen test kit, the detection result can be get in 10-15min. Sampling the specimen with different swab (oropharyngeal swab, nasopharyngeal swab anterior nasal swab), there are different test process that should be .


Allow the test, specimen, buffer and/or controls to reach room temperature (15-30°C or 59-86℉) prior to testing.

1.   Preparation:Open the cap ofextraction tube, transfer the extraction buffer into the extraction tube.

2.   Sample Collection:Use the NP/OP swab provided in the kit to wipetheback wall of the nasopharyngealcavity deep in the nasalcavity (NP swab), or swab overthe lateraland posterior walls of pharynx, as wellas the intratonsillarcleft (OP swab).

3.   Sample Extraction:Insert the swab into the tube,slowly rotate the swab for mixing of the sample and the extraction buffer.Pinch the tube when removing the swab,put the used swab into biohazard bag,and then close the extraction tube with dropper.

4.   Assay procedure:Remove the test cassette from the foil pouch, place the cassette on a clean and level surface .Reverse the specimen extraction tube, holding the specimen extraction tube upright, transfer 3 drops (approximately 80pL) to the specimen well (S) of the test cassette, then start the timer. Interpretation is available within 10-15 minutes.Wait for the colored line(s) to appear. Do not interpret the result after 20 minutes.


Positive(+): Two colored lines appear. One colored line should always appear in the control region (C) and another apparent colored line adjacent should be in the test region (T).

*NOTE: The intensity of the color in the test line region may vary depending on the concentration of SARS-CoV-2 antigen present in the specimen. Therefore, any shade of color In the testline region should be considered positive.

NEGATIVE(-): One colored line appears in the control line region (C). No line appears in the test region(T).

INVALID: Control line fails to appear. Insufficient specimen volume or incorrect procedural techniques are the most likely reasons for control line failure. Review the procedure and repeat the test with a new test. If the problem persists, discontinue using the test kit Immediately and contact your local distributor.

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