• The 5 key invisible performance indicators of Viral Transport Medium Sep. 29. 2020
    The quality of the Viral Transport Tube is not only the "visible" performance indicators, but also the "invisible" performance indicators: 1. "Visible" performance indicators: Directly observed quality indicators such as Viral Transport Tube material, process level, sealing performance, and filling accuracy. 2. "Invisible" performance indicators: include the preservation effect of viral nucleic acid, the preservation effect of the minimum detection limit, microbial indicators, virus inactivation efficiency, etc. These indicators are the key to determining the detection of viral nucleic acid. Preservation effect of viral nucleic acid of Viral Transport Medium: The main function of Viral Transport Medium is to maintain the integrity of viral RNA nucleic acid and inhibit nucleic acid cleavage. Temperature, transportation conditions, and storage days all have an impact on the integrity of RNA. The better the RNA protection, the more accurate the nucleic acid detection of the virus. Virus inactivation efficiency of Viral Transport Medium: The inactivated Viral Transport Medium can improve the safety during sample collection and transportation. Minimum detection limit preservation effect: of Viral Transport Medium: Ensure that samples with low viral loads are detected and false negative test results do not occur. Microbiological indicators of Viral Transport Medium: The presence of microorganisms will accelerate the degradation of viral RNA and affect the sensitivity of nucleic acid detection. The Viral Transport Medium in the Viral Transport Tube is added with the color indicator phenol red. Under normal circumstances, the liquid should be alkaline and the color is purple. Once the microorganisms in the Viral Transport Tube are not up to standard, their reproduction will cause the pH value of the liquid to drop, and the preservation solution will turn yellow.
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  • Why choose nylon flocked swab for viral nucleic acid detection sample collection Sep. 21. 2020
    The flocking technology of nylon flocked swab The disposable sampling nylon flocked swab is made by vertically spraying nylon fluff on the ABS rod coated with flocking glue by flocking technology. The collected samples can be completely and quickly released into the sample preservation solution for viral nucleic acid detection. The advantages of nylon flocked swab High adsorption: The "adsorption" efficiency of nylon flocked swab is 20%~60% higher than other swabs. Rapid collection: The flocked nylon swab can absorb and collect samples in only 3 seconds to 11 seconds; while the sampling time of cotton swabs is as long as 15 seconds to 120 seconds. High release: A domestic pathogen collection experiment found that the release efficiency of flocked nylon swab to pathogens after collection was almost similar to that of the positive control group without swabs at different dilution concentrations; while the release efficiency of pathogens from cotton swabs after collection was obvious On the low side, and the results are more affected by the dilution concentration.
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  • The main components of Viral Transport Medium Aug. 15. 2020
    The components of the Viral Transport Medium include guanidine salt, EDTA, phenol red, buffer solution, etc. The main functions of Viral Transport Medium is to inactivate the virus, crack the protein shell of the virus, and preserve the nucleic acid. Guanidine salt: The most important ingredient in Viral Transport Medium is guanidine salt. Commonly used guanidine salts include guanidine isothiocyanate and guanidine hydrochloride. The guanidine salt can destroy the protein shell on the surface of the virus, release nucleic acid, can be used for nucleic acid detection to confirm whether is infected. The Viral Transport Medium can also inactivate the virus, avoiding the risk of spreading during the sampling, transportation and detection process. EDTA: The second Viral Transport Medium component is EDTA component (Ethylene diamine tetraacetic acid), the main function is to inhibit the degradation of nucleic acid, improve the quality of nucleic acid detection, and ensure the accuracy of subsequent virus amplification. Buffer: The main function of the buffer is to maintain the acidity and alkalinity (pH value) in the tube Phenol red: The acid-base indicator "phenol red" is the reason why the Viral Transport Medium liquid is red. The indicator is red when neutral, purple when the solution becomes alkaline, and yellow when acidic. If the Viral Transport Medium deteriorates, or is contaminated by bacteria, the pH value changes, and it will change from red to yellow. 'Red' indicates that the Viral Transport Medium is within the validity period. At the same time, when the nucleic acid testing personnel add the sample to the colorless sample well, the red color can also allow the testing personnel to observe whether the addition is wrong or whether there is any omission.
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