Flocked nylon swab are commonly used as a tool for sample collection. In order to ensure the accuracy and reliability of test results, it is necessary to standardize the testing standards for flocked nylon swab. Generally, the testing standards for flocked nylon swab should include the following aspects: Appearance quality: The swab should be free of loose fibers, foreign objects, and contamination. Size specifications: The size of the swab should meet the requirements, generally 9cm or 15cm, with a breakpoint of 3cm or 5cm. Water absorption performance: The flocked nylon swabshould have good water absorption performance, which is conducive to the rapid absorption of samples. Packaging inspection: Hot composite peel strength and leakage tests should be carried out on the paper-plastic bag of the swab to ensure the sterilization performance of the individually packaged swab. The above are the basic requirements for the factory testing of flocked nylon swab, ensuring that flocked nylon swab meet the standard requirements to ensure the accuracy and reliability of test results.

1. What is a nylon flocked swab? ChatGPT: Nylon flocked swab is a type of medical swab used for sample collectionfor testing, typically in the fields of molecular biology, virology and bacteriology. 2. What makes flocked swabs different from traditional cotton swabs? ChatGPT: Nylon flocked swabs have a unique surface consisting of short, dense nylon fibers that can effectively collect and retain larger amounts of sample compared to traditional cotton swabs. 3. What are the benefits of using nylon flocked swabs? ChatGPT: Nylon flocked swabs have higher sample recovery rates, improved specimen preservation, and reduced risk of contamination compared to traditional cotton swabs. 4. How are nylon flocked swabs used in sample collection and testing? ChatGPT: Nylon flocked swabs are used to collect a sample from a patient, such as mucus from the nose or throat, and then transported to a laboratory for testing. 5. What types of samples can be collected with a nylon flocked swab? ChatGPT: Nylon flocked swabs can be used to collect a variety of biological samples, including nasopharyngeal, oropharyngeal, or vaginal swabs for COVID-19 testing. 6. What are the storage and transport requirements for nylon flocked swabs? ChatGPT: Nylon flocked swabs should be stored in a dry and cool environment and transported to the laboratory as soon as possible after sample collection to maintain the integrity of the sample. 7. Are nylon flocked swabs approved by regulatory bodies such as CE? ChatGPT: Yes, nylon flocked swabs are approved by regulatory bodies such as CE for use in sample collection and testing. 8. What are the potential drawbacks of using nylon flocked swabs? ChatGPT: The cost of nylon flocked swabs can be higher compared to traditional cotton swabs. 9. How are nylon flocked swabs manufactured? ChatGPT: Nylon flocked swabs are manufactured by depositing short, dense nylon fibers onto a swab shaft through a process called electrostatic flocking. 10. Are there any special considerations for the disposal of used nylon flocked swabs? ChatGPT: Used nylon flocked swabs should be handled and disposed of as potentially infectious medical waste, following guidelines from the relevant local and national health organizations. vincemmed

Compared to common cotton swabs, nylon flocked swab can collect and release more Pathogen samples. 1. High sample adsorption efficiency: Compared to ordinary cotton swabs, nylon flocked swab have the advantages of better adsorption efficiency and short collection. It is difficult to squeeze out water after the cotton swab absorbs water. If the cotton swab is used for nucleic acid detection, not only the water absorption rate is insufficient, but also it is difficult to release the Pathogen sample after collection. Pathogen collection usually needs to be completed in a short time, using nylon flocked swab only needs to be sampled for 3~11 seconds, while cotton swabs take 15~120 seconds, which will greatly increase people's detection time. 2. High sample release efficiency: In addition to being good at capturing and adsorbing when collecting with nylon flocked swab, it is also necessary to fully release the collected pathogens in the preservation solution after sampling. The nylon flocked swab adopts flocking technology, compared with cotton swab, the flocking structure of the nylon flocked swab ensures the full release of the pathogens sample.

After collecting virus sample, usually the sample can not be detected in time, it is necessary to use Viral Transport Medium, for different detection purposes, different Viral Transport Medium need to be used, and in the different virus detection experimental conditions, it is divided into inactivated Viral Transport Medium andcultured Viral Transport Medium. Inactivated Viral Transport Medium Inactivated Viral Transport Medium contain highly effective viral lysate, usually using guanidine salts or other effective viral inactivators, which rapidly inactivate the virus after sampling and release viral nucleic acids for easy detection. At the same time, the inactivated Viral Transport Medium can protect the collector well, which is safe and reliable for the collector and the transportation process, and effectively prevents the risk of secondary infection. Cultured Viral Transport Medium The Cultured Viral Transport Medium does not contain lysate, but a variety of solution components that are conducive to the culture of viral host cells, ensuring the integrity of the virus and helping to increase the survival time and infection stability of the virus. The sample provided by Cultured Viral Transport Medium is basically a live virus, which can maintain the originality of the sample to the greatest extent, and can be used not only for nucleic acid extraction and detection of the virus, but also for the culture and isolation of the virus. However, when used for detection, it should be noted that nucleic acid extraction and purification must be performed after inactivation. Advantages of inactivated Viral Transport Medium Safer: Inactivated Viral Transport Medium contains a variety of virus inactivation components, which effectively inactivates viruses and ensures the safety of transportation and testing personnel. More stable: The Viral Transport Medium contains RNase inhibitory components to ensure the integrity of nucleic acids. Eliminate false negative results. More convenient: Supporting flocking swab, reasonable design, not only increases the safety of the operator but also ensures stable adsorption and release of samples, compared with traditional cotton swab sampling, the same operation can collect more samples at one time.

Collection Procedure of Nylon Flocked Nasopharyngeal Swab Instruct the patient to remove the mask and remove the secretions at the front of the nasal cavity; Open the Nylon Flocked Nasopharyngeal Swab package. Instruct the patient to tilt his head gently back to make it easier for the Nylon Flocked Nasopharyngeal Swab to pass through the nasal passage. Gently insert the Nylon Flocked Nasopharyngeal Swab vertically into one nostril and slowly push the Nylon Flocked Nasopharyngeal Swab forward along the nasal septum until resistance is felt. If it is difficult to push, exit and adjust the angle before pushing. Leave the Nylon Flocked Nasopharyngeal Swab standing for a few seconds (or rotate it in place for several times) to fully absorb the mucus, and then slowly return the Nylon Flocked Nasopharyngeal Swab while rotating the handle. Ask the patient to put on the face mask again. How can I tell if a Nylon Flocked Nasopharyngeal Swab has actually probed the posterior pharyngeal wall? Estimate Depth: Before opening the Nylon Flocked Nasopharyngeal Swab package, estimate the distance between the nostril and the anterior end of the auricle as the depth of the Nylon Flocked Nasopharyngeal Swab entering the nasal passage when it touches the posterior pharyngeal wall. According to this depth, traces can be left in the corresponding position on the Nylon Flocked Nasopharyngeal Swab handle in advance, which is convenient for reference during operation. When resistance is felt in advance: If you feel resistance when pushing the Nylon Flocked Nasopharyngeal Swab before reaching the estimated depth, you can retreat slightly, change the angle slightly to bring the Nylon Flocked Nasopharyngeal Swab closer to the lower wall of the nasal passage, and try to push forward again. Reference - CDC guidance on specimen collection for COVID-19 testing (last updated on 2020.04.29) Link: https://www.cdc.gov/coronavirus/2019-ncov/lab/guidelines-clinical-specimens.html

Nucleic Acid Testing Antigen Detection Detection of samples Nasopharyngeal nylon flocked swab or oropharyngeal nylon flocked swab, sputum nasopharyngeal nylon flocked swab or anterior nasal nylon flocked swab, Sputum or pulmonary Detection target Genes: RdRp, Orflab, and E 、N 、S Structural proteins : S, N, M, and E Detection window period Early stage of infection, until eliminate the virus after recovery Early stage of infection, until eliminate the virus after recovery Detection Time 2-4 Hours Within 15 minutes Detection requirement At least BSL-2 laboratory No special requirements Advantages & Disadvantages Advantages: High sensitivity,high specificity ; Disadvantages: Time-consuming and labor intensive Advantages: easy operation, rapid test time, low cost, the environmental requirements are not high ; Disadvantages: Samples with low levels of virus may not be detected. It is best to use nucleic acid detection methods to check and confirm to avoid misjudgment.

Nasopharyngeal swab have some advantages over oropharyngeal swab: Nasopharyngeal swab can stay in the pharynx for a long time in order to obtain more samples, which is why the positive rate of nasopharyngeal swab is higher than that of oropharyngeal swab. the exposure risk of the Nasopharyngeal swab sampler is lower than that of the oropharyngeal swab, because we can stand behind the patient when sampling, and require the patient to pull down the mask to expose only the nostrils and cover the mouth, and there is no need to look directly at the patient's oral cavity. And there is almost no pharyngeal reflex with Nasopharyngeal swab, a few patients may have sneeze reflex after sampling, and the patient can be covered with elbows or paper towels, and because the sampler is not directly in front of the patient. The exposurerisk of Nasopharyngeal swab is relatively lower, reducing the psychological pressure of the samplers. The disadvantage of the Nasopharyngeal swab is that the patients with oral and pharyngeal swabs have greater psychological fear, and the samplers have more professional training. Whether it is to collect nasopharyngeal swab or oropharyngeal swab, the depth of collection and the duration of contact with the mucosa are the key points. If the nasopharyngeal swab is not collected in the depths of the nasopharyngeal cavity, the patient has a strong vomiting reaction when collecting the oropharyngeal swab, which leads to insufficient sampling time. At this time, most of the cells that may be collected are virus-free cells, which may cause "false negative". This may also be one of the reasons why patients with negative nucleic acid test appear nucleic acid positive again during reexamination. In a chinese study, NPA and OPS methods were compared, SARS, MERS and H1N1 respiratory sampling literatures were reviewed, and it was concluded that among all upper respiratory tract sampling methods, Nasopharyngeal swab and oropharyngeal swab (NS+OPS) were the least harmful to medical staff.

Pharynx: Deep in the nose and mouth, it can be divided into: nasopharynx, oropharynx, and laryngopharynx. When doing nucleic acid, the most commonly sampled areas are the nasopharynx and oropharynx. The reason is that when the virus enters the respiratory tract, very little virus remains in the outer locations, and most of it accumulates in the nasopharynx and oropharynx, which are inside the respiratory tract. Why use anterior nasal swab antigenself-test sampling? Anterior nasal swab is widely use in antigen self-test, because the length of anterior nasal swab is suitable for selftest, besides, although there are few viruses in the nasal cavity, there are more secretions, while in the oral cavity exhalation is large, swallowing is frequent, leaving less virus, there may be false negative test results appear. Therefore, anterior nasal swabs are more commonly used than anterior pharyngeal swabs for antigen self-testing In the case of patients with chronic or allergic rhinitis, it is recommended to blow the nose before anterior nasal swab sampling, but do not wash the nose with saline to avoid affecting the concentration of virus in the nasal cavity, resulting in affecting the sensitivity of the test. In addition, collection of oropharyngeal swab instead of anterior nasal swabis recommended if there is a history and risk factors associated with. Patients with severe allergic rhinitis. Those with malformations in the nasal cavity, such as curvature of the nasal septum. Those with recent symptoms of nasal bleeding. Those who have undergone nasal surgery for not more than 3 months. Those with blood disorders or long-term use of anticoagulant drugs.

The quality of the Viral Transport Tube is not only the "visible" performance indicators, but also the "invisible" performance indicators: 1. "Visible" performance indicators: Directly observed quality indicators such as Viral Transport Tube material, process level, sealing performance, and filling accuracy. 2. "Invisible" performance indicators: include the preservation effect of viral nucleic acid, the preservation effect of the minimum detection limit, microbial indicators, virus inactivation efficiency, etc. These indicators are the key to determining the detection of viral nucleic acid. Preservation effect of viral nucleic acid of Viral Transport Medium: The main function of Viral Transport Medium is to maintain the integrity of viral RNA nucleic acid and inhibit nucleic acid cleavage. Temperature, transportation conditions, and storage days all have an impact on the integrity of RNA. The better the RNA protection, the more accurate the nucleic acid detection of the virus. Virus inactivation efficiency of Viral Transport Medium: The inactivated Viral Transport Medium can improve the safety during sample collection and transportation. Minimum detection limit preservation effect: of Viral Transport Medium: Ensure that samples with low viral loads are detected and false negative test results do not occur. Microbiological indicators of Viral Transport Medium: The presence of microorganisms will accelerate the degradation of viral RNA and affect the sensitivity of nucleic acid detection. The Viral Transport Medium in the Viral Transport Tube is added with the color indicator phenol red. Under normal circumstances, the liquid should be alkaline and the color is purple. Once the microorganisms in the Viral Transport Tube are not up to standard, their reproduction will cause the pH value of the liquid to drop, and the preservation solution will turn yellow.

The flocking technology of nylon flocked swab The disposable sampling nylon flocked swab is made by vertically spraying nylon fluff on the ABS rod coated with flocking glue by flocking technology. The collected samples can be completely and quickly released into the sample preservation solution for viral nucleic acid detection. The advantages of nylon flocked swab High adsorption: The "adsorption" efficiency of nylon flocked swab is 20%~60% higher than other swabs. Rapid collection: The flocked nylon swab can absorb and collect samples in only 3 seconds to 11 seconds; while the sampling time of cotton swabs is as long as 15 seconds to 120 seconds. High release: A domestic pathogen collection experiment found that the release efficiency of flocked nylon swab to pathogens after collection was almost similar to that of the positive control group without swabs at different dilution concentrations; while the release efficiency of pathogens from cotton swabs after collection was obvious On the low side, and the results are more affected by the dilution concentration.